Human TNFRSF10D cDNA Clone in Mammalian Expression Vector
This is a Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 4000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3393879
Supplier Product No.: sc117708
$452.43
Plus shipping costs $50.00
10 μg
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Quick Overview for Human TNFRSF10D cDNA Clone in Mammalian Expression Vector (ABIN3393879)
Gene
DcR2 (TNFRSF10D)
(Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117708
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TNFRSF10D is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Poly(ADP-ribose) polymerase-13 and RNA regulation in immunity and cancer." in: Trends in molecular medicine, Vol. 21, Issue 6, pp. 373-84, (2015) (PubMed).
: "PARP13 regulates cellular mRNA post-transcriptionally and functions as a pro-apoptotic factor by destabilizing TRAILR4 transcript." in: Nature communications, Vol. 5, pp. 5362, (2014) (PubMed).
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: "Poly(ADP-ribose) polymerase-13 and RNA regulation in immunity and cancer." in: Trends in molecular medicine, Vol. 21, Issue 6, pp. 373-84, (2015) (PubMed).
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- DcR2 (TNFRSF10D) (Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))
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Alternative Name
- TNFRSF10D
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Background
- The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_003840, NP_003831
Target
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