Human INPP4A cDNA Clone in Mammalian Expression Vector
This is a Inositol Polyphosphate-4-Phosphatase, Type I, 107kDa plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 3400 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3394007
Supplier Product No.: sc310038
$851.40
Plus shipping costs $50.00
10 μg
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Quick Overview for Human INPP4A cDNA Clone in Mammalian Expression Vector (ABIN3394007)
Gene
INPP4A
(Inositol Polyphosphate-4-Phosphatase, Type I, 107kDa (INPP4A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc310038
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human INPP4A is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3400 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Loss-of-function of inositol polyphosphate-4-phosphatase reversibly increases the severity of allergic airway inflammation." in: Nature communications, Vol. 3, pp. 877, (2012) (PubMed).
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- INPP4A (Inositol Polyphosphate-4-Phosphatase, Type I, 107kDa (INPP4A))
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Alternative Name
- INPP4A
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Background
- This gene encodes an Mg++ independent enzyme that hydrolyzes the 4-position phosphate from the inositol ring of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-bisphosphate. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Aug 2008].Transcript Variant: This variant (a) encodes the shortest isoform (a).
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NCBI Accession
- NM_004027, NP_004018
Target
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