Human TRPM2 cDNA Clone in Mammalian Expression Vector
This is a Transient Receptor Potential Cation Channel, Subfamily M, Member 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL6. Insert length: 5600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3394104
Supplier Product No.: sc303292
$1,273.14
Plus shipping costs $50.00
10 μg
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Quick Overview for Human TRPM2 cDNA Clone in Mammalian Expression Vector (ABIN3394104)
Gene
TRPM2
(Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL6
Promoter
Enhanced CMV Promoter, SP6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc303292
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TRPM2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Four Ca2+ ions activate TRPM2 channels by binding in deep crevices near the pore but intracellularly of the gate." in: The Journal of general physiology, Vol. 133, Issue 2, pp. 189-203, (2009) (PubMed).
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- TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
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Alternative Name
- TRPM2
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Background
- The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2016].Transcript Variant: This variant (1) is the protein-coding variant.
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NCBI Accession
- NM_003307, NP_003298
Target
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