Mouse GM5136 shRNA in Retroviral Vector
Quick Overview for Mouse GM5136 shRNA in Retroviral Vector (ABIN3540874)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Mouse
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Supplier Product No.
- tr508545
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Mouse (Murine) EG368203.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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![Mouse GM5136 shRNA in Retroviral Vector (GFP tag)]()
Mouse GM5136 shRNA in Retroviral Vector (GFP tag)shRNA RNAi Retroviral Vector pGFP-V-RS Kanamycin U6 Promoter GFP tag Transient
![Mouse GM5136 shRNA in Lentiviral Vector (GFP tag)]()
Mouse GM5136 shRNA in Lentiviral Vector (GFP tag)shRNA RNAi Lentiviral Vector pGFP-C-shLenti Chloramphenicol U6 Promoter GFP tag Transient
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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- GM5136 (Predicted Gene 5136 (GM5136))
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Alternative Name
- EG368203
Target
-
