Mouse RAPGEF3 shRNA in Retroviral Vector
This is a Rap Guanine Nucleotide Exchange Factor (GEF) 3 plasmid from OriGene - one-time cited - with shRNA insert cloned into Retroviral VectorpRS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3545133
Supplier Product No.: tr512817
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 16 to 17 Business Days
Quick Overview for Mouse RAPGEF3 shRNA in Retroviral Vector (ABIN3545133)
Gene
RAPGEF3
(Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pRS
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Mouse
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Supplier Product No.
- tr512817
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Mouse (Murine) Rapgef3.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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: "Pancreatic β-cell response to increased metabolic demand and to pharmacologic secretagogues requires EPAC2A." in: Diabetes, Vol. 62, Issue 8, pp. 2796-807, (2013) (PubMed).
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: "Pancreatic β-cell response to increased metabolic demand and to pharmacologic secretagogues requires EPAC2A." in: Diabetes, Vol. 62, Issue 8, pp. 2796-807, (2013) (PubMed).
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- RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))
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Alternative Name
- Rapgef3
Target
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