Human HPSE shRNA in Retroviral Vector
This is a Heparanase plasmid from OriGene - 4 times cited - with shRNA insert cloned into Retroviral VectorpRS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3649115
Supplier Product No.: tr307138
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human HPSE shRNA in Retroviral Vector (ABIN3649115)
Gene
HPSE
(Heparanase (HPSE))
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pRS
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- tr307138
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human HPSE.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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: "Heparanase: A Potential New Factor Involved in the Renal Epithelial Mesenchymal Transition (EMT) Induced by Ischemia/Reperfusion (I/R) Injury." in: PLoS ONE, Vol. 11, Issue 7, pp. e0160074, (2016) (PubMed).
: "Everolimus-induced epithelial to mesenchymal transition in immortalized human renal proximal tubular epithelial cells: key role of heparanase." in: Journal of translational medicine, Vol. 11, pp. 292, (2014) (PubMed).
: "Heparanase and syndecan-1 interplay orchestrates fibroblast growth factor-2-induced epithelial-mesenchymal transition in renal tubular cells." in: The Journal of biological chemistry, Vol. 287, Issue 2, pp. 1478-88, (2012) (PubMed).
: "Heparanase alters arterial structure, mechanics, and repair following endovascular stenting in mice." in: Circulation research, Vol. 104, Issue 3, pp. 380-7, (2009) (PubMed).
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: "Heparanase: A Potential New Factor Involved in the Renal Epithelial Mesenchymal Transition (EMT) Induced by Ischemia/Reperfusion (I/R) Injury." in: PLoS ONE, Vol. 11, Issue 7, pp. e0160074, (2016) (PubMed).
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- HPSE (Heparanase (HPSE))
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Alternative Name
- HPSE
Target
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