Human FZD2 shRNA in Retroviral Vector
This is a Frizzled Family Receptor 2 plasmid from OriGene - 5 times cited - with shRNA insert cloned into Retroviral VectorpRS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Ampicillin.
1 image
(5 references)
Catalog No. ABIN3654824
Supplier Product No.: tr312884
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human FZD2 shRNA in Retroviral Vector (ABIN3654824)
Gene
FZD2
(Frizzled Family Receptor 2 (FZD2))
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pRS
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
-
-
Species
- Human
-
Supplier Product No.
- tr312884
-
Supplier
- OriGene
-
Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human FZD2.
-
Brand
- HuSH-29™
-
Specificity
-
- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
-
Characteristics
-
- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
-
Components
-
- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
-
Selectable Marker
- Puromycin
-
-
-
-
Application Notes
-
- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- 4 °C/-20 °C
-
Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
-
-
-
: "Gastric cancer cell proliferation is suppressed by frizzled-2 short hairpin RNA." in: International journal of oncology, Vol. 46, Issue 3, pp. 1018-24, (2015) (PubMed).
: "Suppression of hepatocellular carcinoma cell proliferation by short hairpin RNA of frizzled 2 with Sonazoid-enhanced irradiation." in: International journal of oncology, Vol. 48, Issue 1, pp. 123-9, (2015) (PubMed).
: "Short hairpin RNA of frizzled-2 suppresses the proliferation of hepatocellular carcinoma cells." in: Oncology letters, Vol. 8, Issue 4, pp. 1519-1522, (2014) (PubMed).
: "Frizzled-2: A potential novel target for molecular pancreatic cancer therapy." in: Oncology letters, Vol. 7, Issue 1, pp. 74-78, (2013) (PubMed).
: "Identification of predictive markers of response to the MEK1/2 inhibitor selumetinib (AZD6244) in K-ras-mutated colorectal cancer." in: Molecular cancer therapeutics, Vol. 9, Issue 12, pp. 3351-62, (2010) (PubMed).
-
-
- FZD2 (Frizzled Family Receptor 2 (FZD2))
-
Alternative Name
- FZD2
Target
-
You are here:
