Human CD44 shRNA in Retroviral Vector
This is a CD44 plasmid from OriGene - 4 times cited - with shRNA insert cloned into Retroviral VectorpRS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3656015
Supplier Product No.: tr314080
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human CD44 shRNA in Retroviral Vector (ABIN3656015)
Gene
CD44
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pRS
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- tr314080
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human CD44.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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: "Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 46, pp. 102-13, (2015) (PubMed).
: "CD44 proteolysis increases CREB phosphorylation and sustains proliferation of thyroid cancer cells." in: Cancer research, Vol. 72, Issue 6, pp. 1449-58, (2012) (PubMed).
: "Stimulation of TLRs by LMW-HA induces self-defense mechanisms in vaginal epithelium." in: Immunology and cell biology, Vol. 89, Issue 5, pp. 630-9, (2011) (PubMed).
: "Neutrophil migration across intestinal epithelium: evidence for a role of CD44 in regulating detachment of migrating cells from the luminal surface." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 11, pp. 7026-36, (2010) (PubMed).
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- CD44
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Alternative Name
- CD44
Target
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