Human HDAC1 shRNA in Retroviral Vector (GFP tag)
This is a Histone Deacetylase 1 plasmid from OriGene - 3 times cited - with shRNA insert cloned into Retroviral VectorpGFP-V-RS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Kanamycin.
1 image
(3 references)
Catalog No. ABIN3737339
Supplier Product No.: tg312496
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human HDAC1 shRNA in Retroviral Vector (GFP tag) (ABIN3737339)
Gene
HDAC1
(Histone Deacetylase 1 (HDAC1))
Fusion tag
GFP tag
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pGFP-V-RS
Promoter
U6 Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- tg312496
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human HDAC1.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA in pGFPC-shLenti vector, 4 unique constructs per gene, 5 ug per vial.
- HuSH 29-mer Scrambled in pGFP-C-shLenti 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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: "Transcriptional Regulation of JARID1B/KDM5B Histone Demethylase by Ikaros, Histone Deacetylase 1 (HDAC1), and Casein Kinase 2 (CK2) in B-cell Acute Lymphoblastic Leukemia." in: The Journal of biological chemistry, Vol. 291, Issue 8, pp. 4004-18, (2016) (PubMed).
: "Histone deacetylase 2 cell autonomously suppresses excitatory and enhances inhibitory synaptic function in CA1 pyramidal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 14, pp. 5924-9, (2013) (PubMed).
: "Nuclear localization of the transcriptional regulator MIER1α requires interaction with HDAC1/2 in breast cancer cells." in: PLoS ONE, Vol. 8, Issue 12, pp. e84046, (2013) (PubMed).
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: "Transcriptional Regulation of JARID1B/KDM5B Histone Demethylase by Ikaros, Histone Deacetylase 1 (HDAC1), and Casein Kinase 2 (CK2) in B-cell Acute Lymphoblastic Leukemia." in: The Journal of biological chemistry, Vol. 291, Issue 8, pp. 4004-18, (2016) (PubMed).
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- HDAC1 (Histone Deacetylase 1 (HDAC1))
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Alternative Name
- HDAC1
Target
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