Human TP53 shRNA in Retroviral Vector (GFP tag)
This is a Tumor Protein P53 plasmid from OriGene - 3 times cited - with shRNA insert cloned into Retroviral VectorpGFP-V-RS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Kanamycin.
1 image
(3 references)
Catalog No. ABIN3744349
Supplier Product No.: tg320558
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human TP53 shRNA in Retroviral Vector (GFP tag) (ABIN3744349)
Gene
p53 (TP53)
(Tumor Protein P53 (TP53))
Fusion tag
GFP tag
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pGFP-V-RS
Promoter
U6 Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient, Stable
-
-
Species
- Human
-
Supplier Product No.
- tg320558
-
Supplier
- OriGene
-
Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human TP53.
-
Brand
- HuSH-29™
-
Specificity
-
- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
-
Characteristics
-
- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
-
Components
-
- Gene-specific shRNA in pGFPC-shLenti vector, 4 unique constructs per gene, 5 ug per vial.
- HuSH 29-mer Scrambled in pGFP-C-shLenti 5 ug plasmid DNA.
-
Selectable Marker
- Puromycin
-
-
-
-
Application Notes
-
- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- 4 °C/-20 °C
-
Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
-
-
-
: "Avian Reovirus Protein p17 Functions as a Nucleoporin Tpr Suppressor Leading to Activation of p53, p21 and PTEN and Inactivation of PI3K/AKT/mTOR and ERK Signaling Pathways." in: PLoS ONE, Vol. 10, Issue 8, pp. e0133699, (2015) (PubMed).
: "Deacetylation of p53 induces autophagy by suppressing Bmf expression." in: The Journal of cell biology, Vol. 201, Issue 3, pp. 427-37, (2013) (PubMed).
: "Gene expression profiles resulting from stable loss of p53 mirrors its role in tissue differentiation." in: PLoS ONE, Vol. 8, Issue 11, pp. e82494, (2013) (PubMed).
-
-
- p53 (TP53) (Tumor Protein P53 (TP53))
-
Alternative Name
- TP53
Target
-
You are here:
