Human DICER1 shRNA in Retroviral Vector
This is a Dicer 1, Ribonuclease Type III plasmid from OriGene - 3 times cited - with shRNA insert cloned into Retroviral VectorpRS. Insert length: not_set. Transient, Stable expression. Suitable for RNAi.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3770568
Supplier Product No.: tr304991
$874.17
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human DICER1 shRNA in Retroviral Vector (ABIN3770568)
Gene
DICER1
(Dicer 1, Ribonuclease Type III (DICER1))
Application
RNA Interference (RNAi)
Insert
shRNA
Vector
Retroviral Vector
Vector Backbone
pRS
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Supplier Product No.
- tr304991
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Supplier
- OriGene
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Purpose
- Pre-designed Hush-29 shRNAs in viral vectors with proven effectiveness for knock-down of Human DICER1.
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Brand
- HuSH-29™
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Specificity
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- The HuSH shRNA gene-specific expression cassettes were optimized to include both the termination signal for RNA Pol III and GC content targeted at 50 % to further improve the quality of the gene-specific shRNA expression vectors.
- One of the four constructs at minimum are guaranteed to produce 70 % or more gene expression knock-down provided a minimum transfection efficiency of 80 % is achieved.
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Characteristics
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- The shRNA gene-specific expression cassettes are prepared using synthetic oligonucleotides.
- These oligonucleotide sequences were computer designed for optimal suppression of gene expression and minimal off-target effects.
- All shRNA sequences are verified through DNA sequencing analysis.
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Components
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- Gene-specific shRNA expression pRS vectors, 5 ug plasmid DNA per vial.
- Four unique constructs per gene.
- HuSH 29-mer NonEffective Scrambled pRS 5 ug plasmid DNA.
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Selectable Marker
- Puromycin
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Application Notes
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- Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection.
- To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples..
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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Storage Comment
- The dried plasmids can be stored at 4°C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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: "Impaired DICER1 function promotes stemness and metastasis in colon cancer." in: Oncogene, Vol. 33, Issue 30, pp. 4003-15, (2014) (PubMed).
: "Aging-induced dysregulation of dicer1-dependent microRNA expression impairs angiogenic capacity of rat cerebromicrovascular endothelial cells." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 68, Issue 8, pp. 877-91, (2013) (PubMed).
: "Dicer-mediated upregulation of BCRP confers tamoxifen resistance in human breast cancer cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6510-21, (2011) (PubMed).
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- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
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Alternative Name
- DICER1
Target
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