Human CD63 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a CD63 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 717 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4920222
$99.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 7 Business Days
Quick Overview for Human CD63 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4920222)
Gene
CD63
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
-
-
Species
- Human
-
Purpose
- Expression/transfection ready cDNA ORF clone of Human CD63 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
-
Sequence
- ATGGCGGTGG AAGGAGGAAT GAAATGTGTG AAGTTCTTGC TCTACGTCCT CCTGCTGGCC TTTTGCGCCT GTGCAGTGGG ACTGATTGCC GTGGGTGTCG GGGCACAGCT TGTCCTGAGT CAGACCATAA TCCAGGGGGC TACCCCTGGC TCTCTGTTGC CAGTGGTCAT CATCGCAGTG GGTGTCTTCC TCTTCCTGGT GGCTTTTGTG GGCTGCTGCG GGGCCTGCAA GGAGAACTAT TGTCTTATGA TCACGTTTGC CATCTTTCTG TCTCTTATCA TGTTGGTGGA GGTGGCCGCA GCCATTGCTG GCTATGTGTT TAGAGATAAG GTGATGTCAG AGTTTAATAA CAACTTCCGG CAGCAGATGG AGAATTACCC GAAAAACAAC CACACTGCTT CGATCCTGGA CAGGATGCAG GCAGATTTTA AGTGCTGTGG GGCTGCTAAC TACACAGATT GGGAGAAAAT CCCTTCCATG TCGAAGAACC GAGTCCCCGA CTCCTGCTGC ATTAATGTTA CTGTGGGCTG TGGGATTAAT TTCAACGAGA AGGCGATCCA TAAGGAGGGC TGTGTGGAGA AGATTGGGGG CTGGCTGAGG AAAAATGTGC TGGTGGTAGC TGCAGCAGCC CTTGGAATTG CTTTTGTCGA GGTTTTGGGA ATTGTCTTTG CCTGCTGCCT CGTGAAGAGT ATCAGAAGTG GCTACGAGGT GATGTAG
-
Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
-
Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
-
Grade
- End-sequenced
-
Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
-
Insert Length
- 717 bp
-
Selectable Marker
- Neomycin
-
Sequencing Primer
-
- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT/-20 °C
-
Storage Comment
-
- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
-
Expiry Date
- 12 months
-
-
- CD63
-
Alternative Name
- CD63
-
Background
- The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012].
-
Gene ID
- 967
-
NCBI Accession
- NM_001780
Target
-
You are here:
