Human SNX6 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Sorting Nexin 6 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 1257 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4923252
$319.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SNX6 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4923252)
Gene
SNX6
(Sorting Nexin 6 (SNX6))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human SNX6 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGCGCGCCT GCGCCGGCCC TCGCCTCGGA GCAGCCATGA TGGAAGGCCT GGACGACGGC CCGGACTTCC TCTCAGAAGA GGACCGCGGA CTTAAAGCAA TAAATGTAGA TCTTCAAAGT GATGCTGCTC TGCAGGTGGA CATTTCTGAT GCTCTTAGTG AGCGGGATAA AGTAAAATTC ACTGTTCACA CAAAGAGTTC ATTGCCAAAT TTTAAACAAA ACGAGTTTTC AGTTGTTCGG CAACATGAGG AATTTATCTG GCTTCATGAT TCCTTTGTTG AAAATGAAGA CTATGCAGGT TATATCATTC CACCAGCACC ACCAAGACCT GATTTTGATG CTTCAAGGGA AAAACTACAG AAGCTTGGTG AAGGAGAAGG GTCAATGACG AAGGAAGAAT TCACAAAGAT GAAACAGGAA CTGGAAGCTG AATATTTGGC AATATTCAAG AAGACAGTTG CGATGCATGA AGTGTTCCTG TGTCGTGTGG CAGCACATCC TATTTTGAGA AGAGATTTAA ATTTCCATGT CTTCTTGGAA TATAATCAAG ATTTGAGTGT GCGAGGAAAA AATAAAAAAG AGAAACTTGA AGACTTCTTT AAAAACATGG TTAAATCAGC AGATGGAGTA ATCGTTTCAG GAGTAAAGGA TGTAGATGAT TTCTTTGAGC ACGAACGAAC ATTTCTTTTG GAGTATCATA ACCGAGTTAA GGATGCATCT GCTAAATCTG ATAGAATGAC AAGATCCCAC AAAAGTGCTG CAGATGATTA CAATAGAATT GGTTCTTCAT TATATGCTTT AGGAACTCAG GATTCTACAG ATATATGCAA GTTTTTTCTC AAAGTTTCAG AACTGTTCGA TAAAACAAGA AAAATAGAAG CACGAGTGTC TGCTGATGAA GACCTCAAAC TTTCTGATCT TTTAAAATAT TACTTAAGAG AATCTCAAGC TGCTAAGGAT CTCCTGTATC GAAGGTCTAG GTCACTAGTG GATTATGAAA ATGCTAATAA AGCACTGGAT AAAGCAAGAG CAAAAAATAA AGATGTTCTA CAGGCCGAAA CTTCCCAACA ATTATGTTGT CAGAAATTTG AAAAAATATC TGAGTCTGCA AAACAAGAAC TTATAGATTT TAAGACAAGA AGAGTTGCTG CATTCAGAAA AAATTTAGTG GAACTGGCAG AGTTAGAACT GAAGCATGCA AAGGGTAATC TACAGTTGCT GCAGAACTGC CTGGCAGTGT TAAATGGAGA CACATAA
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 1257 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- SNX6 (Sorting Nexin 6 (SNX6))
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Alternative Name
- SNX6
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Background
- This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms. [provided by RefSeq, Jul 2008].
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Gene ID
- 58533
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NCBI Accession
- NM_152233
Target
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