Human RLN3 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Relaxin 3 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 330 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4924302
$99.00
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human RLN3 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4924302)
Gene
Relaxin 3 (RLN3)
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human RLN3 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGGCCAGGT ACATGCTGCT GCTGCTCCTG GCGGTATGGG TGCTGACCGG GGAGCTGTGG CCGGGAGCTG AGGCCCGGGC AGCGCCTTAC GGGGTCAGGC TTTGCGGCCG AGAATTCATC CGAGCAGTCA TCTTCACCTG CGGGGGCTCC CGGTGGAGAC GATCAGACAT CCTGGCCCAC GAGGCTATGG AAAAGAACAT TGAGGCTCCA AGAAGTAAAT TTACTTGCTC ACGATTAGAG AAGCTGGATT CATGCTCAGT CAGCCCAGCT CCCAAATGTA CCAGGTCCTC AATTAATAAA GAGAGATACC TTCCCGGATG CAGATGCTGA
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 330 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- Relaxin 3 (RLN3)
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Alternative Name
- RLN3
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Background
- This gene encodes a member of the relaxin family of insulin-like hormones that is expressed predominantly in the brain and plays a role in physiological processes such as stress, memory and appetite regulation. The encoded protein is a precursor that is proteolytically processed to generate a heterodimeric mature form consisting A and B chains interlinked by disulfide bonds. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015].
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Gene ID
- 117579
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NCBI Accession
- NM_001311197
Target
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