Human TMEM48 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Transmembrane Protein 48 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 1905 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4926740
$99.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TMEM48 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4926740)
Gene
TMEM48
(Transmembrane Protein 48 (TMEM48))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human NDC1 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TGATAACCCA GGGCCAGTAC AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 1905 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- TMEM48 (Transmembrane Protein 48 (TMEM48))
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Alternative Name
- NDC1
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Gene ID
- 55706
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NCBI Accession
- NM_001168551
Target
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