Human GHDC ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a GH3 Domain Containing plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 1425 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4929296
$319.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human GHDC ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4929296)
Gene
GHDC
(GH3 Domain Containing (GHDC))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human GHDC with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGCTGCTGT GGCCACTGCT GCTGCTGCTG CTGCTGCTGC CAACATTGGC CCTGCTCAGG CAGCAGCGGT CCCAGGATGC CAGGCTGTCC TGGCTTGCTG GCCTCCAGCA CCGAGTGGCA TGGGGGGCCC TGGTCTGGGC AGCCACCTGG CAGCGGCGGA GGCTGGAGCA GAGCACGCTC CATGTGCACC AGAGCCAGCA GCAGGCCCTG AGGTGGTGTC TACAGGGAGC CCAGCGCCCC CACTGTTCCC TCAGAAGGAG CACAGACATA AGCACCTTCC GGAATCATCT CCCTCTGACC AAGGCCAGCC AGACCCAGCA GGAAGACAGT GGAGAGCAGC CACTGCCCCC GACCTCAAAC CAGGACCTTG GGGAGGCCTC TCTGCAGGCC ACCTTGCTGG GTCTGGCAGC CCTAAACAAG GCCTACCCAG AAGTGCTGGC TCAGGGACGC ACTGCCCGTG TGACGCTTAC ATCCCCTTGG CCCCGACCCC TGCCTTGGCC TGGGAATACC CTGGGCCAGG TGGGCACCCC TGGAACCAAG GACCCTAGGG CCCTGCTGCT GGACGCACTG AGGTCCCCAG GGCTGAGGGC ACTGGAGGCT GGGACGGCTG TCGAACTTCT GGATGTTTTC TTGGGCCTGG AGACTGATGG TGAAGAGCTA GCTGGGGCGA TAGCTGCCGG GAACCCTGGA GCGCCTCTCC GTGAACGGGC AGCTGAGCTC CGGGAGGCCC TAGAGCAGGG GCCACGGGGA CTGGCCCTTC GGCTCTGGCC AAAGCTGCAG GTGGTGGTGA CTCTGGATGC AGGAGGCCAG GCCGAGGCTG TGGCTGCCCT CGGGGCCTTG TGGTGCCAAG GACTAGCCTT CTTCTCTCCT GCTTATGCTG CCTCGGGAGG GGTGCTGGGC CTAAACCTAC AGCCAGAGCA GCCCCATGGG CTCTACCTTC TGCCCCCTGG GGCCCCCTTT ATCGAGCTGC TCCCAGTCAA GGAAGGCACC CAGGAGGAAG CTGCCTCCAC CCTCCTTTTG GCCGAGGCCC AGCAGGGCAA GGAGTATGAG CTGGTGCTGA CGGACCGCGC CAGCCTCACC AGGTGCCGCC TGGGTGATGT GGTGCGAGTG GTTGGTGCCT ACAATCAGTG TCCAGTCGTC AGGTTCATCT GCAGGCTGGA CCAGACCCTG AGTGTGCGAG GGGAAGATAT TGGTGAAGAC CTGTTCTCTG AGGCCCTGGG CCGGGCAGTG GGGCAGTGGG CGGGGGCCAA GCTGCTGGAC CATGGCTGTG TGGAGAGCAG CATTCTGGAG TGGGGAAGAA CAGATAGGGC AGAGGACACT AGACTTGGCA GCTGCCCATT CACCGGAAGT ATGTGTGCAC ATGGGGTCTA TTCACAGATT CCTCTGCGGG CTCTGCTCCC CACTACGAGG TGTTTGTGGC GCTGA
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 1425 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- GHDC (GH3 Domain Containing (GHDC))
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Alternative Name
- GHDC
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Gene ID
- 84514
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NCBI Accession
- NM_001142623
Target
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