Human TEX19 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Testis Expressed 19 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 495 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4934658
$99.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TEX19 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4934658)
Gene
TEX19
(Testis Expressed 19 (TEX19))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human TEX19 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGTGCCCTC CGGTCAGCAT GCGGTATGAG GAAGAGGGCA TGTCCTACCT CTACGCCTCC TGGATGTATC AGCTTCAACA TGGAGATCAG CTAAGCATTT GCTTCACCTG CTTCAAGGCT GCCTTTCTAG ACTTTAAAGA CTTGCTGGAG TCAGAGGACT GGGAAGAAGA CAACTGGGAC CCTGAGCTGA TGGAGCACAC TGAGGCAGAG TCAGAGCAGG AGGGGTCCTC AGGGATGGAG CTGAGCTGGG GGCAGAGCCC AGGACAGCCT GTGCAGGGGG GCTCTGAGGC ATGGGGGCCA GGGACCCTGG CAGCAGCCCC AGAAGGGTTG GAAGATGCAG GTCTGGACCC CCACTTTGTC CCCACTGAAC TATGGCCTCA GGAGGCTGTG CCCCTGGGCC TGGGCCTTGA GGATGCTGAC TGGACCCAGG GTCTTCCCTG GAGATTTGAG GAGCTTCTTA CCTGCTCACA CTGGCCAAGC TTCTTTCCTT CATAG
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 495 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- TEX19 (Testis Expressed 19 (TEX19))
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Alternative Name
- TEX19
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Gene ID
- 400629
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NCBI Accession
- NM_207459
Target
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