Human PAM ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Peptidylglycine alpha-Amidating Monooxygenase plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 2601 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4943020
$679.00
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10 μg
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Quick Overview for Human PAM ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4943020)
Gene
PAM
(Peptidylglycine alpha-Amidating Monooxygenase (PAM))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human PAM with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGGCTGGCC GCGTCCCTAG CCTGCTAGTT CTCCTTGTTT TTCCAAGCAG CTGTTTGGCT TTCCGAAGCC CACTTTCTGT CTTTAAGAGG TTTAAAGAAA CTACCAGACC ATTTTCCAAT GAATGTCTTG GTACCACCAG ACCCGTAGTT CCTATTGATT CATCAGATTT TGCATTGGAT ATTCGCATGC CTGGGGTTAC ACCTAAACAG TCCGATACAT ACTTCTGCAT GTCTATGCGA ATACCAGTGG ATGAGGAAGC CTTCGTGATT GACTTCAAGC CTCGAGCCAG CATGGATACT GTCCATCACA TGTTACTTTT TGGATGCAAT ATGCCTTCAT CCACTGGAAG TTACTGGTTT TGTGATGAAG GAACCTGTAC AGATAAAGCC AATATTCTGT ATGCCTGGGC GAGAAATGCT CCCCCTACCC GGCTCCCCAA AGGTGTTGGA TTCAGAGTTG GAGGAGAGAC TGGAAGTAAA TACTTTGTAC TACAGGTACA CTATGGGGAT ATTAGTGCTT TTAGAGATAA TAACAAGGAC TGTTCTGGTG TGTCCTTACA CCTCACACGT CTGCCACAGC CTTTAATTGC TGGCATGTAC CTTATGATGT CTGTTGACAC TGTTATCCCA GCAGGAGAAA AAGTGGTGAA TTCTGACATT TCATGCCATT ATAAAAATTA TCCAATGCAT GTCTTTGCCT ATAGAGTTCA CACTCACCAT TTAGGTAAGG TAGTAAGTGG ATACAGAGTA AGAAATGGAC AGTGGACACT GATTGGACGG CAGAGCCCTC AGCTGCCACA GGCTTTCTAC CCTGTGGGGC ATCCAGTTGA TGTAAGTTTT GGTGACCTAC TGGCTGCAAG ATGTGTATTC ACTGGTGAAG GAAGGACAGA AGCCACACAC ATTGGTGGCA CGTCTAGTGA TGAAATGTGC AACTTATACA TTATGTATTA CATGGAAGCC AAGCATGCAG TTTCTTTCAT GACCTGTACC CAGAATGTAG CTCCAGATAT GTTCAGAACC ATACCACCAG AGGCCAACAT TCCAATTCCC GTGAAGTCTG ATATGGTTAT GATGCATGAA CATCATAAAG AAACAGAATA TAAAGATAAG ATTCCTTTAC TACAGCAGCC AAAACGAGAA GAAGAAGAAG TGTTAGACCA GGATTTCCAC ATGGAAGAGG CACTGGATTG GCCTGGAGTA TACTTGTTAC CAGGCCAGGT TTCTGGGGTG GCTCTAGACC CTAAGAATAA CCTGGTGATT TTCCACAGAG GTGACCATGT CTGGGATGGA AACTCGTTTG ACAGCAAGTT TGTTTACCAG CAAATAGGAC TCGGACCAAT TGAAGAAGAC ACTATTCTTG TCATAGATCC AAATAATGCT GCAGTACTCC AGTCCAGTGG AAAAAATCTG TTTTACTTGC CACATGGCTT GAGTATAGAT AAAGATGGGA ATTATTGGGT CACAGACGTG GCTCTCCATC AGGTGTTCAA ACTGGATCCA AACAATAAAG AAGGCCCTGT ATTAATCCTG GGAAGGAGCA TGCAACCAGG CAGTGACCAG AATCACTTCT GTCAACCCAC TGATGTGGCT GTGGATCCAG GCACTGGAGC CATTTATGTA TCAGATGGTT ACTGCAACAG CAGGATTGTG CAGTTTTCAC CAAGTGGAAA GTTCATCACA CAGTGGGGAG AAGAGTCTTC AGGGAGCAGT CCTCTGCCAG GCCAGTTCAC TGTTCCTCAC AGCTTGGCTC TTGTGCCTCT TTTGGGCCAA TTATGTGTGG CAGACCGGGA AAATGGTCGG ATCCAGTGTT TTAAAACTGA CACCAAAGAA TTTGTGAGAG AGATTAAGCA TTCATCATTT GGAAGAAATG TATTTGCAAT TTCATATATA CCAGGCTTGC TCTTTGCAGT GAATGGGAAG CCTCATTTTG GGGACCAAGA ACCTGTACAA GGATTTGTGA TGAACTTTTC CAATGGGGAA ATTATAGACA TCTTCAAGCC AGTGCGCAAG CACTTTGATA TGCCTCATGA TATTGTTGCA TCTGAAGATG GGACTGTGTA CATTGGAGAT GCTCATACCA ACACCGTGTG GAAGTTCACC TTGACTGAGA AATTGGAACA TCGATCAGTT AAAAAGGCTG GCATTGAGGT CCAGGAAATC AAAGAAGCCG AGGCAGTTGT TGAAACCAAA ATGGAGAACA AACCCACCTC CTCAGAATTG CAGAAGATGC AAGAGAAACA GAAACTGATC AAAGAGCCAG GCTCGGGAGT GCCTGTTGTT CTCATTACAA CCCTTCTGGT TATTCCGGTG GTTGTCCTGC TGGCCATTGC CATATTTATT CGGTGGAAAA AATCAAGGGC CTTTGGAGAT TCTGAACACA AACTCGAGAC GAGTTCAGGA AGAGTACTGG GAAGATTTAG AGGAAAGGGA AGTGGAGGCT TAAACCTTGG TAATTTCTTT GCAAGCCGTA AGGGCTACAG TCGAAAAGGG TTTGACCGGC TTAGCACTGA GGGCAGTGAC CAAGAGAAAG AGGATGATGG AAGTGAATCA GAAGAGGAGT ATTCAGCACC TCTGCCTGCG CTCGCACCTT CCTCCTCCTG A
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 2601 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
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Alternative Name
- PAM
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Background
- This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq, Jul 2008].
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Gene ID
- 5066
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NCBI Accession
- NM_138821
Target
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