Human ARMC4 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
This is a Armadillo Repeat Containing 4 plasmid with ORF insert cloned into Mammalian Expression VectorpcDNA3.1+C-(K)-DYK. Insert length: 2211 bp. Transient, Stable expression. Suitable for PExp.
Bacterial selection: Ampicillin. Grade: End-sequenced.
Catalog No. ABIN4944569
$559.00
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ARMC4 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag) (ABIN4944569)
Gene
ARMC4
(Armadillo Repeat Containing 4 (ARMC4))
Fusion tag
DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pcDNA3.1+C-(K)-DYK
Promoter
CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Species
- Human
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Purpose
- Expression/transfection ready cDNA ORF clone of Human ARMC4 with C terminal DYKDDDDK tag is ideal for express proteins in E.coli & mammalian cells.
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Sequence
- ATGTCTAAAT TGGGAATTAG CTTCAGTGAA GACCAGCAAA AGGAAAAGGA TCAGCTTGGC AAAGCCCCCA AGAAGGAAGA AGCAGCTGCC CTCCGCAAAG ACATTTCTGG TTCAGACAAA AGGTCACTGG AGAAGAACCA AATTAATTTT TGGAGGAATC AAATGACCAA GAGATGGGAA CCAAGCTTAA ACTGGAAGAC CACTGTTAAT TACAAAGGCA AAGGCTCAGC AAAAGAAATC CAAGAGGACA AACACACAGG AAAACTTGAA AAACCAAGAC CATCTGTTTC ACACGGAAGA GCACAATTAC TTCGGAAGAG TGCTGAAAAG ATTGAGGAAA CTGTTAGCGA TAGCTCCTCA GAAAGTGAGG AAGATGAAGA ACCACCTGAC CATCGTCAGG AAGCAAGTGC AGATTTGCCA TCAGAATATT GGCAAATTCA GAAGCTGGTG AAATATTTAA AGGGAGGAAA TCAAACAGCT ACAGTGATTG CGTTGTGTTC AATGAGGGAT TTCAGCTTAG CTCAAGAAAC CTGCCAGTTG GCCATCAGAG ATGTTGGAGG CCTGGAAGTG CTGATAAATT TGCTTGAAAC CGATGAAGTC AAATGTAAGA TTGGTTCATT AAAAATACTG AAGGAAATCA GTCATAATCC TCAAATCAGA CAGAATATTG TTGACCTTGG GGGCTTACCA ATTATGGTGA ATATACTTGA TTCTCCACAC AAGAGTCTAA AATGTTTGGC AGCCGAGACT ATCGCGAATG TTGCCAAGTT TAAAAGAGCA CGGCGGGTGG TGAGGCAGCA CGGGGGTATC ACCAAACTGG TTGCTCTACT AGACTGTGCA CATGATTCCA CAAAACCTGC CCAATCGAGT CTGTATGAGG CCAGAGACGT GGAAGTGGCT CGCTGTGGGG CACTGGCCCT GTGGAGCTGC AGTAAGAGTC ATACGAATAA AGAAGCCATC CGCAAAGCTG GGGGCATTCC TCTGTTGGCT CGGCTGCTGA AGACTTCTCA TGAAAACATG CTAATTCCAG TGGTGGGGAC ATTGCAAGAG TGTGCATCAG AGGAAAACTA CCGGGCTGCA ATCAAAGCAG AAAGGATCAT TGAAAACCTT GTCAAGAACC TAAATAGTGA GAATGAGCAG CTGCAGGAGC ACTGCGCCAT GGCCATTTAC CAGTGTGCTG AAGATAAGGA AACCCGGGAC CTCGTTAGGC TGCACGGAGG ACTTAAGCCC TTGGCCAGTC TACTCAATAA CACTGACAAT AAAGAGCGGT TAGCTGCTGT CACAGGGGCT ATATGGAAAT GTTCCATCAG CAAAGAGAAT GTTACCAAGT TTCGGGAATA CAAAGCCATT GAAACCTTGG TGGGACTTCT AACAGATCAG CCTGAAGAAG TACTTGTGAA TGTGGTTGGG GCCTTGGGAG AATGCTGCCA AGAACGTGAA AACCGAGTCA TTGTCCGGAA ATGTGGTGGC ATTCAACCAC TTGTGAACCT CCTTGTTGGA ATAAACCAAG CTCTTCTTGT GAATGTTACA AAAGCAGTTG GTGCTTGTGC AGTAGAACCT GAAAGTATGA TGATAATTGA TCGCTTAGAT GGAGTTCGTT TGTTGTGGTC CCTGCTGAAA AATCCTCACC CAGACGTGAA GGCCAGCGCA GCATGGGCAC TCTGTCCATG CATCAAAAAT GCAAAGGATG CTGGGGAAAT GGTTCGTTCC TTTGTTGGTG GTTTGGAACT TATTGTCAAT TTACTGAAAT CAGATAACAA AGAAGTTCTG GCAAGTGTAT GTGCTGCCAT TACCAACATA GCAAAAGATC AAGAAAATTT AGCTGTTATC ACAGATCATG GAGTTGTTCC TTTATTGTCC AAACTGGCAA ATACAAATAA CAATAAATTG AGACATCATC TAGCAGAAGC TATTTCACGT TGCTGTATGT GGGGCAGGAA TAGAGTGGCC TTCGGTGAGC ACAAAGCAGT GGCTCCACTA GTGCGTTATC TGAAATCAAA TGACACCAAC GTGCATCGGG CGACAGCTCA GGCCTTGTAC CAACTCTCAG AAGACGCCGA TAACTGCATC ACCATGCATG AGAATGGTGC AGTAAAGCTT CTACTGGATA TGGTTGGGTC CCCTGACCAG GATCTCCAGG AAGCTGCAGC TGGTTGTATA TCCAATATCC GCAGGCTGGC TCTTGCTACA GAGAAGGCAA GATACACTTG A
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Specificity
- ORF Insert Method: CloneEZ® Seamless cloning technology, recombination-based cloning technology
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Characteristics
- Gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gene cDNA ORF which is encoded by the open reading frame (ORF) sequence.
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Grade
- End-sequenced
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Components
- The GenEZ ORF clone is delivered as 10 μg of lyophilized plasmid DNA in a vial.
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Insert Length
- 2211 bp
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Selectable Marker
- Neomycin
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Sequencing Primer
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- Forward primer: 5'-TAATACGACTCACTATAGGG-3'
- Reverse primer: 5'-CCTCGACTGTGCCTTCTA-3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT/-20 °C
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Storage Comment
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- Keep the vial sealed and store at -20°C for long-term storage.
- Before use, centrifuge the vial at 6,000 g x g for 1 minute at 4°C.
- Open the lid and add 100 μl (or other volume depending on your desired final concentration) of distilled water (or TE buffer) to dissolve the DNA.
- If necessary, heat the solution at 50°C for 15 minutes to dissolve the DNA.
- Close the lid and vortex the vial for 1 minute.
- Aliquot the dissolved plasmid DNA and store in small aliquots at -20°C.
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Expiry Date
- 12 months
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- ARMC4 (Armadillo Repeat Containing 4 (ARMC4))
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Alternative Name
- ARMC4
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Background
- The protein encoded by this gene contains ten Armadillo repeat motifs (ARMs) and one HEAT repeat, and is thought to be involved in ciliary and flagellar movement. This protein has been shown to localize to the ciliary axonemes and at the ciliary base of respiratory cells. Studies indicate that mutations in this gene cause partial outer dynein arm (ODA) defects in respiratory cilia. The cilia of cells with mutations in this gene displayed either reduced ciliary beat frequency and amplitude, or, complete immotility. Some individuals with primary ciliary dyskensia (PCD) have been shown to have mutations in this gene. PCD is characterized by chronic airway disease and left/right body asymmetry defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015].
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Gene ID
- 55130
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NCBI Accession
- NM_001312689
Target
-
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