pCasGuide Control Vector
Empty
NC, Clon
Mammalian Expression Vector
pCas-Guide
Ampicillin
U6 Promoter
unconjugated
Transient
Catalog No. ABIN4948178
Quick Overview for pCasGuide Control Vector (ABIN4948178)
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Mammalian Expression Vector
Vector Backbone
pCas-Guide
Promoter
U6 Promoter, Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Purpose
- pCAS-Guide vector (with Cas9 expression) for genomic target sequence cloning
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Specificity
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All-in-one vector
Expresses human codon-optimized Cas9
BamH I and BsmB I can be used for genomic target sequence cloning
Expresses gRNA containing the inserted target sequence and the gRNA scaffold -
Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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