pCMV6-AC Control Vector
Empty
NC, Clon
Mammalian Expression Vector
pCMV6-AC
Ampicillin
Enhanced CMV Promoter
unconjugated
Transient
Catalog No. ABIN4948179
Quick Overview for pCMV6-AC Control Vector (ABIN4948179)
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Purpose
- pCMV6-AC, mammalian vector with non-tagged expression
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Specificity
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This vector is essentially the same as the pCMV6-Entry vector, except for the absence of the C-terminal Myc-DDK tag.
ORF cloned into this vector at SgfI and RsrII sites will express the ORF-encoded protein plus two additional amino acids at the C-terminus, T and R.
The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein. -
Selectable Marker
- Neomycin
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Sequencing Primer
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VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3'
XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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