pCMV6-AC-Myc-DDK Control Vector
Empty
NC, Clon
Mammalian Expression Vector
pCMV6-AC-Myc-DDK
Ampicillin
Enhanced CMV Promoter
Myc-DYKDDDDK Tag
Transient
Catalog No. ABIN4948180
Quick Overview for pCMV6-AC-Myc-DDK Control Vector (ABIN4948180)
Fusion tag
Myc-DYKDDDDK Tag
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-AC-Myc-DDK
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Purpose
- pCMV6-AC-Myc-DDK, mammalian vector with C-terminal Myc-DDK tag
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Specificity
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ORFs cloned in this vector will be expressed as a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
Such clones are the best for detection and purification of the transgene expression using anti-Myc or anti-DDK antibodies
If the C-terminal tagging interferes with the protein's function, you can choose the N-terminal Myc-DDK-tagging vector -
Selectable Marker
- Neomycin
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Sequencing Primer
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VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3'
XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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