pCMV6-Entry Control Vector
Empty
NC, Clon
Mammalian Expression Vector
pCMV6-Entry
Kanamycin
Enhanced CMV Promoter
unconjugated
Transient
Catalog No. ABIN4948181
Quick Overview for pCMV6-Entry Control Vector (ABIN4948181)
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Purpose
- pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag
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Specificity
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ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-DDK antibodies.
Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms.
The native stop codon is present in the cDNA insert, therefore the C-terminal tag won't be expressed -
Selectable Marker
- Neomycin
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Sequencing Primer
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VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3'
XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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