pCMV6-Neo Control Vector
Empty
NC, Clon
Mammalian Expression Vector
pCMV6-Neo
Ampicillin
Enhanced CMV Promoter
unconjugated
Transient
Catalog No. ABIN4948182
Quick Overview for pCMV6-Neo Control Vector (ABIN4948182)
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Neo
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient, Stable
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Purpose
- Cloning vector pCMV6-Neo, circular and NotI-digested / dephosphorylated vector
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Specificity
- A Neomycin resistant version of its pCMV cloning vector as a kit to those customer who wish to create a stable cell line.
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Selectable Marker
- Neomycin
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Sequencing Primer
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VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3'
XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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