pGFP-C-shLenti Control Vector
Empty
NC, Clon
Lentiviral Vector
pGFP-C-shLenti
Chloramphenicol
U6 Promoter
GFP tag
Transient
Catalog No. ABIN4948186
Quick Overview for pGFP-C-shLenti Control Vector (ABIN4948186)
Fusion tag
GFP tag
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Lentiviral Vector
Vector Backbone
pGFP-C-shLenti
Promoter
U6 Promoter
Bacterial Resistance
Chloramphenicol
Expression Type
Transient, Stable
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Purpose
- shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector)
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Specificity
- PGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
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Selectable Marker
- Puromycin
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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