pGFP-V-RS Control Vector
Empty
NC, Clon
Retroviral Vector
pGFP-V-RS
Kanamycin
U6 Promoter
GFP tag
Transient
Catalog No. ABIN4948187
Quick Overview for pGFP-V-RS Control Vector (ABIN4948187)
Fusion tag
GFP tag
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Retroviral Vector
Vector Backbone
pGFP-V-RS
Promoter
U6 Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient, Stable
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Purpose
- shRNA GFP Cloning Vector (pGFP-V-RS Vector)
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Specificity
- The HuSH pGFP-V-RS plasmid vector contains both 5' and 3' LTRs of Moloney murine leukemia virus (MMLV) that flank the puromycin marker and the U6-shRNA expression cassette. Upon transient transfection of the plasmids into a packaging cell line, replication deficient viruses can be obtained and used to infect target cells. The puromycin-N-acetyl transferase gene and Kanamycin gene provide selection of antibiotics puromycin and kanamycin, respectively. There is an integrated turboGFP element driven by a cMV promoter to readily verify transfection efficiency.
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Selectable Marker
- Puromycin
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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