pRFP-C-RS Control Vector
Empty
NC, Clon
Retroviral Vector
pRFP-C-RS
Chloramphenicol
U6 Promoter
RFP tag
Transient
Catalog No. ABIN4948188
Quick Overview for pRFP-C-RS Control Vector (ABIN4948188)
Fusion tag
RFP tag
Application
Negative Control (NC), Cloning (Clon)
Insert
Empty
Vector
Retroviral Vector
Vector Backbone
pRFP-C-RS
Promoter
U6 Promoter
Bacterial Resistance
Chloramphenicol
Expression Type
Transient, Stable
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Purpose
- shRNA RFP Cloning Vector (pRFP-C-RS Vector)
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Specificity
- The HuSH pRFP-C-RS plasmid vector was created with an integrated turboRFP element to readily verify transfection efficiency. It incorporates both a chloramphenicol and puromycin resistance elements for greater selection capabilities. The pRFP-C-RS plasmid is also ideal for monitoring the dual-gene knockdown experiments when used alongside pGFP-V-RS expression plasmid.
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Selectable Marker
- Puromycin
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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1. Briefly centrifuge for 30 seconds.
2. Carefully open the tube and add sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom.
5. Store the plasmid at -20 °C. -
Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized plasmid can be stored at ambient temperature for three months.
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