Mouse DHFR CRISPR gRNA in Lenti Particles
This is a Dihydrofolate Reductase plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5081146
$693.75
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
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United States
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Quick Overview for Mouse DHFR CRISPR gRNA in Lenti Particles (ABIN5081146)
Gene
Dihydrofolate Reductase (DHFR)
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Mouse
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Purpose
- Individual gRNA against Dhfr in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- Sequence available upon placing order
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Dhfr
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- Dihydrofolate Reductase (DHFR)
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Alternative Name
- Dhfr
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NCBI Accession
- NM_010049
Target
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