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Human ACTA2 CRISPR gRNA in Lenti Particles

This is a Actin, alpha 2, Smooth Muscle, Aorta plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN. Bacterial selection: Ampicillin.
Catalog No. ABIN5083492
$693.75
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
Shipping to: United States
Delivery in 31 to 41 Business Days

Quick Overview for Human ACTA2 CRISPR gRNA in Lenti Particles (ABIN5083492)

Gene

Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Application

Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)

Insert

gRNA

Vector

Lentiviral Vector

Vector Backbone

pLenti-U6-sgRNA-PGK-Neo

Promoter

U6 Promoter, PGK Promoter

Bacterial Resistance

Ampicillin

Expression Type

Stable, Transient
  • Species

    Human

    Purpose

    Individual gRNA against ACTA2 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)

    Sequence

    Sequence available upon placing order

    Specificity

    GRNAs are designed for use with Cas9 Nuclease only.

    Components

    Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of ACTA2

    Selectable Marker

    Neomycin
  • Application Notes

    Recommended for quality control: Restriction Enzyme Digest and Sequencing

    Restrictions

    For Research Use only
  • Format

    Viral Particles

    Storage

    -80 °C

    Expiry Date

    12 months
  • Target

    Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

    Alternative Name

    ACTA2

    NCBI Accession

    NM_001141945
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