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Rat BCKDHA CRISPR gRNA in Lenti Particles

gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Catalog No. ABIN5084216

Quick Overview for Rat BCKDHA CRISPR gRNA in Lenti Particles (ABIN5084216)

Gene

BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Application

Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)

Insert

gRNA

Vector

Lentiviral Vector

Vector Backbone

pLenti-U6-sgRNA-PGK-Neo

Promoter

U6 Promoter, PGK Promoter

Bacterial Resistance

Ampicillin

Expression Type

Stable, Transient
  • Species

    Rat

    Purpose

    Individual gRNA against Bckdha in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)

    Sequence

    Sequence available upon placing order

    Specificity

    GRNAs are designed for use with Cas9 Nuclease only.

    Components

    Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Bckdha

    Selectable Marker

    Neomycin
  • Application Notes

    Recommended for quality control: Restriction Enzyme Digest and Sequencing

    Restrictions

    For Research Use only
  • Format

    Viral Particles

    Storage

    -80 °C

    Expiry Date

    12 months
  • Target

    BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

    Alternative Name

    Bckdha

    NCBI Accession

    NM_012782
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