Human TNF alpha CRISPR gRNA in Lenti Particles
This is a Tumor Necrosis Factor alpha plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5108350
$666.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
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United States
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Quick Overview for Human TNF alpha CRISPR gRNA in Lenti Particles (ABIN5108350)
Gene
TNF alpha
(Tumor Necrosis Factor alpha (TNF alpha))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Human
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Purpose
- Individual gRNA against TNF in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- ""43 GAGGCGCTCC CCAAGAAGAC ""
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of TNF
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- TNF
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NCBI Accession
- NM_000594
Target
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