Rat CDKN1B CRISPR gRNA in Lenti Particles
gRNA
PExp, GEEN
Lentiviral Vector
pLenti-U6-sgRNA-PGK-Neo
Ampicillin
U6 Promoter
unconjugated
Stable
Catalog No. ABIN5113074
Quick Overview for Rat CDKN1B CRISPR gRNA in Lenti Particles (ABIN5113074)
Gene
CDKN1B
(Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Rat
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Purpose
- Individual gRNA against Cdkn1b in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- ""171 CGAAATTCCA CTTGCGC""
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Cdkn1b
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Alternative Name
- Cdkn1b
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NCBI Accession
- NM_031762
Target
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