Mouse PPP1R1B CRISPR gRNA in Lenti Particles
gRNA
PExp, GEEN
Lentiviral Vector
pLenti-U6-sgRNA-PGK-Neo
Ampicillin
U6 Promoter
unconjugated
Stable
Catalog No. ABIN5117418
Quick Overview for Mouse PPP1R1B CRISPR gRNA in Lenti Particles (ABIN5117418)
Gene
DARPP32 (PPP1R1B)
(Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Mouse
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Purpose
- Individual gRNA against Ppp1r1b in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- Sequence available upon placing order
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Ppp1r1b
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
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Alternative Name
- Ppp1r1b
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NCBI Accession
- NM_144828
Target
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