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Rat LEF1 CRISPR gRNA in Lenti Particles

gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Catalog No. ABIN5120408

Quick Overview for Rat LEF1 CRISPR gRNA in Lenti Particles (ABIN5120408)

Gene

LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Application

Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)

Insert

gRNA

Vector

Lentiviral Vector

Vector Backbone

pLenti-U6-sgRNA-PGK-Neo

Promoter

U6 Promoter, PGK Promoter

Bacterial Resistance

Ampicillin

Expression Type

Stable, Transient
  • Species

    Rat

    Purpose

    Individual gRNA against Lef1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)

    Sequence

    Sequence available upon placing order

    Specificity

    GRNAs are designed for use with Cas9 Nuclease only.

    Components

    Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Lef1

    Selectable Marker

    Neomycin
  • Application Notes

    Recommended for quality control: Restriction Enzyme Digest and Sequencing

    Restrictions

    For Research Use only
  • Format

    Viral Particles

    Storage

    -80 °C

    Expiry Date

    12 months
  • Target

    LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

    Alternative Name

    Lef1

    NCBI Accession

    NM_130429
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