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Rat PNMT CRISPR gRNA in Lenti Particles

This is a Phenylethanolamine N-Methyltransferase plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN. Bacterial selection: Ampicillin.
Catalog No. ABIN5121732
$693.75
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
Shipping to: United States
Delivery in 31 to 41 Business Days

Quick Overview for Rat PNMT CRISPR gRNA in Lenti Particles (ABIN5121732)

Gene

PNMT (Phenylethanolamine N-Methyltransferase (PNMT))

Application

Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)

Insert

gRNA

Vector

Lentiviral Vector

Vector Backbone

pLenti-U6-sgRNA-PGK-Neo

Promoter

U6 Promoter, PGK Promoter

Bacterial Resistance

Ampicillin

Expression Type

Stable, Transient
  • Species

    Rat

    Purpose

    Individual gRNA against Pnmt in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)

    Sequence

    Sequence available upon placing order

    Specificity

    GRNAs are designed for use with Cas9 Nuclease only.

    Components

    Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Pnmt

    Selectable Marker

    Neomycin
  • Application Notes

    Recommended for quality control: Restriction Enzyme Digest and Sequencing

    Restrictions

    For Research Use only
  • Format

    Viral Particles

    Storage

    -80 °C

    Expiry Date

    12 months
  • Target

    PNMT (Phenylethanolamine N-Methyltransferase (PNMT))

    Alternative Name

    Pnmt

    NCBI Accession

    NM_031526
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