Mouse ATP5F1 CRISPR gRNA in Lenti Particles
gRNA
PExp, GEEN
Lentiviral Vector
pLenti-U6-sgRNA-PGK-Neo
Ampicillin
U6 Promoter
unconjugated
Stable
Catalog No. ABIN5127582
Quick Overview for Mouse ATP5F1 CRISPR gRNA in Lenti Particles (ABIN5127582)
Gene
ATP5F1
(ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Mouse
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Purpose
- Individual gRNA against Atp5f1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- Sequence available upon placing order
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Atp5f1
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))
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Alternative Name
- Atp5f1
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NCBI Accession
- NM_009725
Target
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