Human AXL CRISPR gRNA in Lenti Particles
This is a AXL Receptor tyrosine Kinase plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5130727
$1,511.06
Plus shipping costs $50.00, if applicable $20.00 dry ice
3 x 300 μL
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Quick Overview for Human AXL CRISPR gRNA in Lenti Particles (ABIN5130727)
Gene
AXL
(AXL Receptor tyrosine Kinase (AXL))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Human
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Purpose
- Set of 3 gRNA against AXL in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
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sgAXL-1-a: GACCAGGTGG AGGTTACG
sgAXL-1-b: GCCAACCCAC GGAGCTGG
sgAXL-2-a: GATGCTTGCG AGGTGAG
sgAXL-2-b: GCCCCATCAG CTTCGGCT
sgAXL-3-a: GAACAGGGTA CCCTGCAG
sgAXL-3-b: GTACCGGCTG GCGTATCA -
Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of AXL
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- AXL (AXL Receptor tyrosine Kinase (AXL))
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Alternative Name
- AXL
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NCBI Accession
- NM_001699
Target
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