Mouse RB1 CRISPR gRNA in Lenti Particles
This is a Retinoblastoma 1 plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5149984
$1,450.62
Plus shipping costs $50.00, if applicable $20.00 dry ice
3 x 300 μL
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Quick Overview for Mouse RB1 CRISPR gRNA in Lenti Particles (ABIN5149984)
Gene
Retinoblastoma 1 (RB1)
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Mouse
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Purpose
- Set of 3 gRNA against Rb1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
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""184 CTTTCTCTGA CATGATC
393 AATCGATACC AGTACCA
733 CCTCTTCTGG GTGTTCG"" -
Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of Rb1
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- Retinoblastoma 1 (RB1)
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Alternative Name
- Rb1
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NCBI Accession
- NM_009029
Target
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