Human SPI1 CRISPR gRNA in Lenti Particles
This is a Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 plasmid with gRNA insert cloned into Lentiviral VectorpLenti-U6-sgRNA-PGK-Neo. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5150919
$693.75
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
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Quick Overview for Human SPI1 CRISPR gRNA in Lenti Particles (ABIN5150919)
Gene
SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-PGK-Neo
Promoter
U6 Promoter, PGK Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Human
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Purpose
- Individual gRNA against SPI1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (Cas9 required separately)
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Sequence
- ""54 AGACCTGGTG CCCTATGACA ""
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Specificity
- GRNAs are designed for use with Cas9 Nuclease only.
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Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of SPI1
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Selectable Marker
- Neomycin
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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Alternative Name
- SPI1
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NCBI Accession
- NM_003120
Target
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