Human AKT1 CRISPR gRNA + Cas9 in Lenti Particles
This is a V-Akt Murine Thymoma Viral Oncogene Homolog 1 plasmid with gRNA + Cas9 insert cloned into Lentiviral VectorpLenti-U6-sgRNA-SFFV-Cas9-2A-Puro. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5197765
$1,968.75
Plus shipping costs $50.00, if applicable $20.00 dry ice
3 x 300 μL
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Quick Overview for Human AKT1 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5197765)
Gene
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Promoter
U6 Promoter, SFFV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Human
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Purpose
- Set of 3 gRNA against AKT1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
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""6 CGACGTGGCT ATTGTGA
87 AGGTGCCATC ATTCTTG
156 CCACAGAGAA GTTGTTG"" -
Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of AKT1
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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Alternative Name
- AKT1
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NCBI Accession
- NM_001014431
Target
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