Human CA125 CRISPR gRNA + Cas9 in Lenti Particles
Quick Overview for Human CA125 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5208317)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Purpose
- Set of 3 gRNA against MUC16 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
- ""45 TGCTCCCTGT CATCAAGGAG 126 GACATCTACA GGTGCAATCG 196 AGTCCTACAT CTTCGGTTGT ""
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Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of MUC16
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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![Human CA125 shRNA in Retroviral Vector]()
Human CA125 shRNA in Retroviral VectorshRNA RNAi Retroviral Vector pRS Ampicillin U6 Promoter unconjugated Transient
![Human CA125 shRNA in Retroviral Vector (RFP tag)]()
Human CA125 shRNA in Retroviral Vector (RFP tag)shRNA RNAi Retroviral Vector pRFP-C-RS Chloramphenicol U6 Promoter RFP tag Transient
![Human CA125 shRNA in Retroviral Vector (GFP tag)]()
Human CA125 shRNA in Retroviral Vector (GFP tag)shRNA RNAi Retroviral Vector pGFP-V-RS Kanamycin U6 Promoter GFP tag Transient
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
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Alternative Name
- MUC16
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NCBI Accession
- NM_024690
Target
-
