Rat PHLPP1 CRISPR gRNA + Cas9 in Lenti Particles
This is a PH Domain and Leucine Rich Repeat Protein Phosphatase 1 plasmid with gRNA + Cas9 insert cloned into Lentiviral VectorpLenti-U6-sgRNA-SFFV-Cas9-2A-Puro. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5209987
$857.21
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
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Quick Overview for Rat PHLPP1 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5209987)
Gene
PHLPP1
(PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Promoter
U6 Promoter, SFFV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Rat
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Purpose
- Individual gRNA against Phlpp1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
- ""1788 TGCGTTGTGA TGCAACC""
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Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Phlpp1
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))
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Alternative Name
- Phlpp1
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NCBI Accession
- NM_021657
Target
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