Rat ATG7 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9
PExp, GEEN
Lentiviral Vector
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Ampicillin
U6 Promoter
unconjugated
Stable
Catalog No. ABIN5245983
Quick Overview for Rat ATG7 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5245983)
Gene
ATG7
(ATG7 Autophagy Related 7 (ATG7))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Promoter
U6 Promoter, SFFV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Rat
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Purpose
- Individual gRNA against Atg7 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
- ""173 GAACTCCAAT GTCAAGC""
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Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Atg7
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- ATG7 (ATG7 Autophagy Related 7 (ATG7))
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Alternative Name
- Atg7
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NCBI Accession
- NM_001012097
Target
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