Human RGS21 CRISPR gRNA + Cas9 in Lenti Particles
Quick Overview for Human RGS21 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5258885)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Purpose
- Individual gRNA against RGS21 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
- Sequence available upon placing order
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Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of RGS21
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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![Human RGS21 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human RGS21 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
![Human RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Rat RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Rat RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Mouse RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse RGS21 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- RGS21 (Regulator of G-Protein Signaling 21 (RGS21))
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Alternative Name
- RGS21
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NCBI Accession
- NM_001039152
Target
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