Mouse PGAM5 CRISPR gRNA + Cas9 in Lenti Particles
This is a Phosphoglycerate Mutase Family Member 5 plasmid with gRNA + Cas9 insert cloned into Lentiviral VectorpLenti-U6-sgRNA-SFFV-Cas9-2A-Puro. Insert length: not_set. Stable, Transient expression. Suitable for PExp and GEEN.
Bacterial selection: Ampicillin.
Catalog No. ABIN5290338
$857.21
Plus shipping costs $50.00, if applicable $20.00 dry ice
300 μL
Shipping to:
United States
Delivery in 31 to 41 Business Days
Quick Overview for Mouse PGAM5 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5290338)
Gene
PGAM5
(Phosphoglycerate Mutase Family Member 5 (PGAM5))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Promoter
U6 Promoter, SFFV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
-
-
Species
- Mouse
-
Purpose
- Individual gRNA against Pgam5 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
-
Sequence
- ""281 GATGTGCCTT GTGGCCT""
-
Specificity
-
GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Pgam5
-
Selectable Marker
- Puromycin
-
Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
-
-
-
-
Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
-
Restrictions
- For Research Use only
-
-
-
Format
- Viral Particles
-
Storage
- -80 °C
-
Expiry Date
- 12 months
-
-
- PGAM5 (Phosphoglycerate Mutase Family Member 5 (PGAM5))
-
Alternative Name
- Pgam5
-
NCBI Accession
- NM_028273
Target
-
You are here:
