Mouse 4933408B17RIK CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9
PExp, GEEN
Lentiviral Vector
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Ampicillin
U6 Promoter
unconjugated
Stable
Catalog No. ABIN5291306
Quick Overview for Mouse 4933408B17RIK CRISPR gRNA + Cas9 in Lenti Particles (ABIN5291306)
Gene
4933408B17Rik (4933408B17RIK)
(RIKEN CDNA 4933408B17 Gene (4933408B17RIK))
Application
Protein Expression (PExp), Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Lentiviral Vector
Vector Backbone
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Promoter
U6 Promoter, SFFV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Stable, Transient
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Species
- Mouse
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Purpose
- Set of 3 gRNA against 4933408B17Rik in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
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Sequence
- Sequence available upon placing order
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Specificity
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GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of 4933408B17Rik
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Selectable Marker
- Puromycin
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Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
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![Mouse 4933408B17RIK cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse 4933408B17RIK cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
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Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
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Restrictions
- For Research Use only
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Format
- Viral Particles
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Storage
- -80 °C
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Expiry Date
- 12 months
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- 4933408B17Rik (4933408B17RIK) (RIKEN CDNA 4933408B17 Gene (4933408B17RIK))
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Alternative Name
- 4933408B17Rik
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NCBI Accession
- NM_177773
Target
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