Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@genomics-online.com

Human HRES1 CRISPR gRNA + Cas9 in Lenti Particles

gRNA + Cas9 GEEN, PExp Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Catalog No. ABIN5292280

Quick Overview for Human HRES1 CRISPR gRNA + Cas9 in Lenti Particles (ABIN5292280)

Gene

HRES1 (HTLV-1 Related Endogenous Sequence (HRES1))

Application

Genome Editing with Engineered Nucleases (GEEN), Protein Expression (PExp)

Insert

gRNA + Cas9

Vector

Lentiviral Vector

Vector Backbone

pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro

Promoter

U6 Promoter, SFFV Promoter

Bacterial Resistance

Ampicillin

Expression Type

Stable, Transient
  • Species

    Human

    Purpose

    Set of 3 gRNA against HRES1 in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)

    Sequence

    Sequence available upon placing order

    Specificity

    GRNAs are designed for use with Cas9 Nuclease only.
    Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells.

    Components

    Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of HRES1

    Selectable Marker

    Puromycin

    Sequencing Primer

    U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
  • Application Notes

    Recommended for quality control: Restriction Enzyme Digest and Sequencing

    Restrictions

    For Research Use only
  • Format

    Viral Particles

    Storage

    -80 °C

    Expiry Date

    12 months
  • Target

    HRES1 (HTLV-1 Related Endogenous Sequence (HRES1))

    Alternative Name

    HRES1

    NCBI Accession

    NM_001007238
You are here: