Human POTEM CRISPR gRNA + Cas9 in Lenti Particles
Quick Overview for Human POTEM CRISPR gRNA + Cas9 in Lenti Particles (ABIN5294214)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
-
-
Species
- Human
-
Purpose
- Set of 3 gRNA against POTEM in Lentiviral Particles with a Titer of >1x10e7 IU/mL. (sgRNA and Cas9 in a single vector)
-
Sequence
- Sequence available upon placing order
-
Specificity
-
GRNAs are designed for use with Cas9 Nuclease only.
Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. -
Components
- Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of POTEM
-
Selectable Marker
- Puromycin
-
Sequencing Primer
- U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
-
-
![Human POTEM ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human POTEM ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
![Human POTEM shRNA in Retroviral Vector (GFP tag)]()
Human POTEM shRNA in Retroviral Vector (GFP tag)shRNA RNAi Retroviral Vector pGFP-V-RS Kanamycin U6 Promoter GFP tag Transient
![Human POTEM shRNA in Retroviral Vector]()
Human POTEM shRNA in Retroviral VectorshRNA RNAi Retroviral Vector pRS Ampicillin U6 Promoter unconjugated Transient
![Human POTEM shRNA in Lentiviral Vector (GFP tag)]()
Human POTEM shRNA in Lentiviral Vector (GFP tag)shRNA RNAi Lentiviral Vector pGFP-C-shLenti Chloramphenicol U6 Promoter GFP tag Transient
![Human POTEM CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human POTEM CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)gRNA + Cas9 GEEN Mammalian Expression Vector pCas-Guide Ampicillin U6 Promoter Myc-DYKDDDDK Tag Transient
-
-
Application Notes
- Recommended for quality control: Restriction Enzyme Digest and Sequencing
-
Restrictions
- For Research Use only
-
-
-
Format
- Viral Particles
-
Storage
- -80 °C
-
Expiry Date
- 12 months
-
-
- POTEM (POTE Ankyrin Domain Family, Member M (POTEM))
-
Alternative Name
- POTEM
-
NCBI Accession
- NM_001145442
Target
-
