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Human GSDMB ORF Clone in Gateway Cloning Vector

ORF Clon Gateway Cloning Vector pDONR223 Spectinomycin unconjugated

Catalog No. ABIN5317024

Quick Overview for Human GSDMB ORF Clone in Gateway Cloning Vector (ABIN5317024)

Gene

Gasdermin B (GSDMB)

Application

Cloning (Clon)

Insert

ORF

Vector

Gateway Cloning Vector

Vector Backbone

pDONR223

Bacterial Resistance

Spectinomycin

Species

Human

Purpose

Entry clone of human cDNA (ORF) without STOP codon in Gateway pDONR223 Vector.

Sequence

ATGTTCAGCG TATTTGAGGA AATCACAAGA ATTGTAGTTA AGGAGATGGA TGCTGGAGGG GATATGATTG CCGTTAGAAG CCTTGTTGAT GCTGATAGAT TCCGCTGCTT CCATCTGGTG GGGGAGAAGA GAACTTTCTT TGGATGCCGG CACTACACAA CAGGCCTCAC CCTGATGGAC ATTCTGGACA CAGATGGGGA CAAGTGGTTA GATGAACTGG ATTCTGGGCT CCAAGGTCAA AAGGCTGAGT TTCAA

Components

2 μg of lyophilized gene in pDONR223 vector

Insert Length

1236 bp

Sequencing Primer

Forward primer M13R (5'→3'): GTAAAACGACGGCCAGT, Reverse primer T7P (5'→3'): TAATACGACTCACTATAGG
Human GSDMB ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human GSDMB cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Human GSDMB CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human GSDMB cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human GSDMB cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human GSDMB cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human GSDMB cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human GSDMB CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human GSDMB CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human GSDMB CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Restrictions

For Research Use only
Format

Lyophilized

Storage

-20 °C
Target

Gasdermin B (GSDMB)

Alternative Name

GSDMB

Background

Gasdermin B

Gene ID

55876

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