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Human GAGE1 ORF Clone in Gateway Cloning Vector

ORF Clon Gateway Cloning Vector pDONR223 Spectinomycin unconjugated

Catalog No. ABIN5319038

Quick Overview for Human GAGE1 ORF Clone in Gateway Cloning Vector (ABIN5319038)

Gene

G Antigen 1 (GAGE1)

Application

Cloning (Clon)

Insert

ORF

Vector

Gateway Cloning Vector

Vector Backbone

pDONR223

Bacterial Resistance

Spectinomycin

Species

Human

Purpose

Entry clone of human cDNA (ORF) without STOP codon in Gateway pDONR223 Vector.

Sequence

ATGAGTTGGC GAGGAAGATC GACCTATTAT TGGCCTAGAC CAAGGCGCTA TGTACAGCCT CCTGAAATGA TTGGGCCTAT GCGGCCCGAG CAGTTCAGTG ATGAAGTGGA ACCAGCAACA CCTGAAGAAG GGGAACCAGC AACTCAACGT CAGGATCCTG CAGCTGCTCA GGAGGGAGAG GATGAGGGAG CATCTGCAGG TCAAGGGCCG AAGCCTGAAG CTGATAGCCA GGAACAGGGT CACCCACAGA CTGGG

Components

2 μg of lyophilized gene in pDONR223 vector

Insert Length

420 bp

Sequencing Primer

Forward primer M13R (5'→3'): GTAAAACGACGGCCAGT, Reverse primer T7P (5'→3'): TAATACGACTCACTATAGG
Human GAGE1 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Human GAGE1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human GAGE1 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human GAGE1 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human GAGE1 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human GAGE1 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human GAGE1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human GAGE1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human GAGE1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human GAGE1 ORF Clone in Mammalian Expression Vector (DYKDDDDK Tag)
ORF PExp Mammalian Expression Vector pcDNA3.1+C-(K)-DYK Ampicillin CMV Promoter DYKDDDDK Tag Transient

Restrictions

For Research Use only
Format

Lyophilized

Storage

-20 °C
Target

G Antigen 1 (GAGE1)

Alternative Name

GAGE1

Background

G antigen 1

Gene ID

2543

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